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BioHPC Cloud:
: User Guide

 


BioHPC Cloud Software

There is 677 software titles installed in BioHPC Cloud. The sofware is available on all machines (unless stated otherwise in notes), complete list of programs is below, please click on a title to see details and instructions. Tabular list of software is available here

Please read details and instructions before running any program, it may contain important information on how to properly use the software in BioHPC Cloud.

454 gsAssembler or gsMapper, a5, ABRicate, ABruijn, ABySS, AdapterRemoval, adephylo, Admixtools, Admixture, agrep, albacore, Alder, AlleleSeq, ALLMAPS, ALLPATHS-LG, AMOS, AMPHORA, amplicon.py, analysis, ANGSD, Annovar, antiSMASH, apollo, Arlequin, aspera, assembly-stats, atac-seq-pipeline, athena_meta, ATLAS, Atlas-Link, ATLAS_GapFill, ATSAS, Augustus, AWS command line interface, axe, BactSNP, bam2fastx, bamtools, bamUtil, Basset, BayeScan, Bayescenv, BBmap, BCFtools, bcl2fastq, BCP, Beagle, Beast2, bedops, BEDtools, bfc, bgc, bgen, bigQF, bigWig, bioawk, biobambam, Bioconductor, biom-format, BioPerl, BioPython, Birdsuite, Bismark, blasr, BLAST, blast2go, BLAT, BLUPF90, BMGE, bmtagger, Boost, Bowtie, Bowtie2, BPGA, Bracken, BRAKER, BRAT-NextGen, BreedingSchemeLanguage, breseq, brocc, BSseeker2, BUSCO, BWA, bwa-meth, cactus, canu, CAP3, CarveMe, cBar, CBSU RNAseq, CCTpack, cd-hit, CEGMA, CellRanger, cellranger-atac, centrifuge, CFSAN SNP pipeline, CheckM, chimera, chromosomer, Circlator, Circos, Circuitscape, CLUMPP, Clustal Omega, CLUSTALW, Cluster, cmake, CNVnator, compat, CONCOCT, Conda, copyNumberDiff, cortex_var, CRISPRCasFinder, CRISPResso, CrossMap, CRT, cuda, Cufflinks, cutadapt, dadi, dadi-1.6.3_modif, danpos, dDocent, DeconSeq, deepTools, defusion, delly, DESMAN, destruct, DETONATE, diamond, diploSHIC, discoal, Discovar, Discovar de novo, distruct, DiTASiC, DIYABC, Docker, dREG, dREG.HD, Drop-seq, dropEst, dropSeqPipe, dsk, Dsuite, ea-utils, ecopcr, ecoPrimers, ectyper, EDGE, edirect, eems, EGAD, EIGENSOFT, EMBOSS, entropy, ephem, epic2, ermineJ, ete3, exabayes, exonerate, eXpress, FALCON, FALCON_unzip, Fast-GBS, fasta, FastANI, fastcluster, FastME, FastML, fastp, FastQ Screen, fastq_pair, fastq_species_detector, FastQC, fastsimcoal26, fastStructure, FastTree, FASTX, feh, fineRADstructure, fineSTRUCTURE, FIt-SNE, flash, flash2, flexbar, Flexible Adapter Remover, Flye, FMAP, FragGeneScan, FragGeneScan, freebayes, FunGene Pipeline, GAEMR, Galaxy, GATK, gatk4, gatk4amplicon.py, Gblocks, GBRS, gcc, GCTA, GDAL, gdc-client, GEM library, GEMMA, geneid, GeneMark, GeneMarker, Genome STRiP, GenomeMapper, GenomeStudio (Illumina), GenomeThreader, GenomicConsensus, gensim, GEOS, germline, gerp++, GET_PHYLOMARKERS, GffCompare, gffread, giggle, GMAP/GSNAP, GNU Compilers, GNU parallel, go-perl, GoShifter, gradle-4.4, graftM, graphviz, GRiD, Grinder, GROMACS, GSEA, GTDB-Tk, GTFtools, Gubbins, GUPPY, HapCompass, HAPCUT, HAPCUT2, hapflk, HaploMerger, Haplomerger2, HapSeq2, HarvestTools, HiC-Pro, HiCExplorer, HISAT2, HMMER, Homer, HOTSPOT, HTSeq, HUMAnN2, hyperopt, HyPhy, iAssembler, IBDLD, IDBA-UD, IDP-denovo, idr, IgBLAST, IGoR, IGV, IMa2, IMa2p, IMAGE, ImageJ, ImageMagick, Immcantation, impute2, IMSA-A, INDELseek, infernal, Infomap, InStruct, InteMAP, InterProScan, ipyrad, IQ-TREE, iRep, jags, java, jbrowse, jellyfish, JoinMap, julia, jupyter, kallisto, Kent Utilities, keras, khmer, KmerFinder, kraken, kSNP, kWIP, LACHESIS, lammps, LAST, lcMLkin, LDAK, leeHom, Lep-MAP3, lftp, Lighter, LINKS, LocARNA, LocusZoom, lofreq, longranger, LS-GKM, LUCY, LUCY2, LUMPY, lyve-SET, MACE, MACS, MaCS simulator, MACS2, MAFFT, mafTools, Magic-BLAST, magick, MAKER, MAQ, MASH, mashtree, Mashtree, MaSuRCA, Mauve, MaxBin, mccortex, mcl, megahit, MeGAMerge, MEGAN, MELT, MEME Suite, MERLIN, MetaBAT, MetaCRAST, metaCRISPR, MetAMOS, MetaPathways, MetaPhlAn, MetaVelvet, MetaVelvet-SL, MGmapper, Migrate-n, mikado, Minimac3, Minimac4, minimap2, mira, miRDeep2, MISO (misopy), MITObim, MiXCR, MixMapper, MKTest, mlst, MMAP, MMSEQ, MMseqs2, monocle3, mosdepth, mothur, MrBayes, mrsFAST, msld, MSMC, msprime, MSR-CA Genome Assembler, msstats, MSTMap, mugsy, MultiQC, multiz-tba, MUMmer, muscle, MUSIC, muTect, Nanopolish, ncftp, Nemo, Netbeans, NEURON, new_fugue, Nextflow, NextGenMap, nf-core/rnaseq, NGS_data_processing, NGSadmix, ngsDist, ngsF, ngsLD, NgsRelate, ngsTools, NGSUtils, NLR-Parser, Novoalign, NovoalignCS, nvidia-docker, Oases, OBITools, OMA, OrthoFinder, Orthomcl, PacBioTestData, PAGIT, paleomix, PAML, pandas, pandaseq, PanPhlAn, Panseq, Parsnp, PASA, PASTEC, PAUP*, pb-assembly, pbalign, pbh5tools, PBJelly, PBSuite, PCAngsd, PeakRanger, PeakSplitter, PEAR, PEER, PennCNV, PfamScan, PGDSpider, ph5tools, Phage_Finder, PHAST, phenopath, Phobius, PHRAPL, PHYLIP, PhyloCSF, phyloFlash, phylophlan, PhyloPhlAn2, PhyML, Picard, pigz, Pilon, Pindel, piPipes, PIQ, PlasFlow, Platypus, plink, plink2, Plotly, popbam, PopCOGenT, Porechop, portcullis, pplacer, PRANK, prinseq, prodigal, progenomics, progressiveCactus, PROJ, prokka, psutil, pyani, PyCogent, pyfaidx, pyGenomeTracks, PyMC, pyopencl, pypy, pyRAD, Pyro4, PySnpTools, python, PyTorch, PyVCF, QIIME, QIIME2 q2cli, QTCAT, Quake, Qualimap, QuantiSNP2, QUAST, QUMA, R, RACA, racon, RADIS, RadSex, RAPTR-SV, RAxML, Ray, rclone, Rcorrector, RDP Classifier, REAPR, Relate, RelocaTE2, RepeatMasker, RepeatModeler, RFMix, rgdal, RGI, Rgtsvm, ripgrep, rJava, RNAMMER, rnaQUAST, Rnightlights, Roary, Rqtl, Rqtl2, RSEM, RSeQC, RStudio, rtfbs_db, sabre, SaguaroGW, salmon, Sambamba, samblaster, sample, SampleTracker, Samtools, Satsuma, Satsuma2, scikit-learn, Scoary, scythe, seaborn, SecretomeP, selscan, Sentieon, SeqPrep, seqtk, Seurat, sf, sgrep, sgrep sorted_grep, SHAPEIT, shore, SHOREmap, shortBRED, SHRiMP, sickle, SignalP, SimPhy, simuPOP, singularity, sistr_cmd, SKESA, skewer, SLiM, smcpp, SMRT Analysis, SMRT LINK, snakemake, snap, SNAPP, snATAC, SNeP, snippy, snp-sites, SnpEff, SNPgenie, SNPhylo, SNPsplit, SNVPhyl, SOAP2, SOAPdenovo, SOAPdenovo-Trans, SOAPdenovo2, SomaticSniper, sorted_grep, SPAdes, SPALN, SparCC, SPARTA, SRA Toolkit, srst2, stacks, Stacks 2, stairway-plot, stampy, STAR, Starcode, statmodels, STITCH, STPGA, StrainPhlAn, strawberry, Strelka, stringMLST, StringTie, STRUCTURE, Structure_threader, supernova, SURPI, sutta, SVDetect, svtools, SWAMP, SweepFinder, sweepsims, tabix, Tandem Repeats Finder (TRF), TargetP, TASSEL 3, TASSEL 4, TASSEL 5, tcoffee, TensorFlow, TEToolkit, tfTarget, TMHMM, tmux, TopHat, Torch, traitRate, Trans-Proteomic Pipeline (TPP), TransComb, TransDecoder, TRANSIT, transrate, TRAP, treeCl, treemix, Trim Galore!, trimmomatic, Trinity, Trinotate, tRNAscan-SE, UCSC Kent utilities, UMAP, UMI-tools, Unicycler, UniRep, unrar, usearch, Variant Effect Predictor, VarScan, VCF-kit, vcf2diploid, vcfCooker, vcflib, vcftools, vdjtools, Velvet, vep, VESPA, vg, ViennaRNA, VIP, viral-ngs, virmap, VirSorter, VirusDetect, VirusFinder 2, VizBin, vmatch, vsearch, vt, WASP, wgs-assembler (Celera), Wise2 (Genewise), Xander_assembler, yaha

Details for GET_PHYLOMARKERS (hide)

Name:GET_PHYLOMARKERS
Version:20190820
OS:Linux
About:Identify optimal genomic markers for phylogenomics, population genetics and genomic taxonomy (includes GET_HOMOLOGUES)
Added:8/20/2019 2:47:54 PM
Updated:
Link:https://github.com/vinuesa/get_phylomarkers
Manual:https://github.com/vinuesa/get_phylomarkers/blob/master/docs/GET_PHYLOMARKERS_manual.md#get_phylomarkers-tutorial
Notes:

The software authors provide a Docker container with all dependencies pre-installed. This includes both GET_PHYLOMARKERS and its companion program GET_HOMOLOGUES. On the BioHPC Cloud the command docker is replaced with docker1.

 

Here are BioHPC-specific instructions to installing and running the GET_PHYLOMARKERS & GET_HOMOLOGUES Docker container:

 

Download and run the get_homologues Docker container, connecting the output folder inside the container to /workdir/${USER}/get_homPhy:

mkdir /workdir/${USER}
docker1 run -d -t -u root -v /workdir/${USER}/get_homPhy:/home/root/get_homPhy docker.io/csicunam/get_homologues:latest /bin/bash
docker1 ps -a

CONTAINER ID: ec48b7aa9df1
IMAGE: docker.io/csicunam/get_homologues:latest
COMMAND: "/bin/bash"
CREATED: About a minute ago
STATUS: Up About a minute
PORTS:
NAMES: jcg233__biohpc_1

Connect to the container and change into the output folder (replace "ec48b7aa9df1" with your CONTAINER ID in all docker1 commands):

docker1 exec -u root -it ec48b7aa9df1 /bin/bash
root@ec48b7aa9df1:/workdir# cd /home/root/get_homPhy

Verify that GET_HOMOLOGUES is properly setup:

root@ec48b7aa9df1:/home/root/get_homPhy# get_homologues.pl -v

Verify that GET_PHYLOMARKERS is properly setup:

root@ec48b7aa9df1:/home/root/get_homPhy# run_get_phylomarkers_pipeline.sh -v

Copy the test sequence data to your working folder inside docker:

root@ec48b7aa9df1:/home/root/get_homPhy# cp -r /get_phylomarkers/test_sequences/ /home/root/get_homPhy/

Run part of the tutorial (run GET_HOMOLOGUES to compute the set of homologous clusters using the BDBH, COGtriangles and OMCL clustering algorithms):

root@ec48b7aa9df1:/home/root/get_homPhy# cd test_sequences/
root@ec48b7aa9df1:/home/root/get_homPhy/test_sequences# get_homologues.pl -d pIncAC -t 12 -e -n 1

To analyze your own data, follow the manual at:
https://github.com/vinuesa/get_phylomarkers/blob/master/docs/GET_PHYLOMARKERS_manual.md#get_phylomarkers-tutorial

When you are done, exit the docker session:

root@ec48b7aa9df1:/home/root/get_homPhy/test_sequences# exit

If you are finished with the running docker container, stop it, then remove it:

docker1 stop ec48b7aa9df1
docker1 rm ec48b7aa9df1

Go to the output folder and claim ownership of the output files:

cd /workdir/${USER}/get_homPhy
ls -l
total 0
drwxr-xr-x 6 root root 98 Aug 20 14:35 test_sequences


docker1 claim
ls -l
total 0
drwxr-xr-x 6 jcg233 root 98 Aug 20 14:35 test_sequences

 

For more information on using Docker in the BioHPC Cloud, see:
https://biohpc.cornell.edu/lab/doc/BioHPC_Docker_WOrkshop_20190422.pdf

 


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